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UBE2T/HSPC150 antibody

Cat.#: 116537

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Product Information

  • Product Name
    UBE2T/HSPC150 antibody
  • Documents
  • Description
    UBE2T/HSPC150 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HeLa cells, K-562 cells. Positive IF detected in HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 23 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    HSPC150 antibody; PIG50 antibody; UBE2T antibody; Ubiquitin carrier protein T antibody; Ubiquitin protein ligase T antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UBE2T/HSPC150 recombinant protein (Accession Number: NM_014176). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IF: 1:50-1:500

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116537(UBE2T antibody) at dilution of 1:800

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116537(UBE2T antibody) at dilution of 1:800

    IP Result of anti-UBE2T (IP:Catalog No:116537, 3ug; Detection:Catalog No:116537 1:500) with HeLa cells lysate 3000ug.

    IP Result of anti-UBE2T (IP:Catalog No:116537, 3ug; Detection:Catalog No:116537 1:500) with HeLa cells lysate 3000ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:116537(UBE2T Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:116537(UBE2T Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
  • References
    • Hao J, Xu A, Xie X. Elevated expression of UBE2T in lung cancer tumors and cell lines. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 29(3):195-203. 2008.
    • Hira A, Yoshida K, Sato K. Mutations in the gene encoding the E2 conjugating enzyme UBE2T cause Fanconi anemia. American journal of human genetics. 96(6):1001-7. 2015.
    • Jacquemont C, Taniguchi T. Proteasome function is required for DNA damage response and fanconi anemia pathway activation. Cancer research. 67(15):7395-405. 2007.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"