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Product Name
UBE2J1 Polyclonal Antibody
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Description
Polyclonal antibody to UBE2J1
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
UBE2J1 antibody; CGI-76 antibody; HSPC153 antibody; HSPC205 antibody; HSU93243 antibody; NCUBE-1 antibody; NCUBE1 antibody; UBC6 antibody; UBC6E antibody; Ubc6p antibody; ubiquitin-conjugating enzyme E2 J1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2J1 (NP_057105.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - UBE2J1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UBE2J1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"