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Product Name
UBE2F antibody
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Description
UBE2F Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, mouse ovary tissue. Positive IHC detected in human skin tissue. Observed molecular weight by Western-blot: 21kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NCE2 antibody; NEDD8 carrier protein UBE2F antibody; NEDD8 conjugating enzyme 2 antibody; NEDD8 protein ligase UBE2F antibody; UBE2F antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBE2F recombinant protein (Accession Number: NM_080678). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116524(UBE2F antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human skin using Catalog No:116524(UBE2F antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:116524(UBE2F antibody) at dilution of 1:100 (under 40x lens)
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Background
UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).
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