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Product Name
UBE2E2 antibody
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Description
UBE2E2 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Positive WB detected in human thyroid gland tissue, human brain tissue, mouse embryo tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ25157 antibody; UBCH8 antibody; UBE2E2 antibody; Ubiquitin carrier protein E2 antibody; Ubiquitin protein ligase E2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UBE2E2 recombinant protein (Accession Number: NM_001370225). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human thyroid gland tissue were subjected to SDS PAGE followed by western blot with Catalog No:116522(UBE2E2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116522(UBE2E2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using UBE2E2 antibody Catalog No:116522 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).
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References
- Coleman KE, Grant GD, Haggerty RA. Sequential replication-coupled destruction at G1/S ensures genome stability. Genes & development. 29(16):1734-46. 2015.
- Shibata E, Abbas T, Huang X, Wohlschlegel JA, Dutta A. Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex. Molecular and cellular biology. 31(15):3136-45. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"