-
Product Name
UBC13 antibody
- Documents
-
Description
UBC13 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in mouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 17 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
BLU antibody; UBC13 antibody; UbcH ben antibody; UBE2N antibody; Ubiquitin carrier protein N antibody; Ubiquitin protein ligase N antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of UBC13 recombinant protein (Accession Number: NM_003348). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:116507(UBE2N antibody) at dilution of 1:1000
IP Result of anti-UBE2N (IP:Catalog No:116507, 4ug; Detection:Catalog No:116507 1:600) with MCF-7 cells lysate 1280ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116507(UBE2N Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116507(UBE2N Antibody) at dilution of 1:200 (under 40x lens)
-
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"