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Product Name
UBASH3B Polyclonal Antibody
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Description
Polyclonal antibody to UBASH3B
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
UBASH3B antibody; STS-1 antibody; STS1 antibody; TULA-2 antibody; TULA2 antibody; p70 antibody; ubiquitin associated and SH3 domain containing B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human UBASH3B (NP_116262.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - UBASH3B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using UBASH3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - UBASH3B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spleen using UBASH3B antibody at dilution of 1:100 (40x lens).
Immunofluorescence - UBASH3B Polyclonal Antibody
Immunofluorescence analysis of A549 cells using UBASH3B antibody . Blue: DAPI for nuclear staining.
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Background
Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"