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Product Name
U2AF35 antibody
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Description
U2AF35 Mouse Monoclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, COLO 320 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 35-40 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp313J1712 antibody; FP793 antibody; RNU2AF1 antibody; U2AF1 antibody; U2AF35 antibody; U2AFBP antibody
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Isotype
Mouse IgG2c
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Preparation
This antibody was obtained by immunization of U2AF35 recombinant protein (Accession Number: NM_006758). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107652(U2AF35 Antibody) at dilution of 1:1000
IP Result of anti-U2AF35 (IP:Catalog No:107652, 3ug; Detection:Catalog No:107652 1:500) with HeLa cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107652(U2AF35 Antibody) at dilution of 1:50 (under 40x lens)
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Background
U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.
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