Human YES1/c-Yes (His & GST Tag) recombinant protein

Human YES1/c-Yes (His & GST Tag) recombinant protein

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Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.

Tyrosine-protein kinase Yes

YES; HST441; YES1; C-YES; AI323763; P61-YES

P07947

YES1; YES

Baculovirus-Insect Cells

A DNA sequence encoding the human YES1 (NP_005424.1) (Gly 2-Leu 543) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human YES1/GST chimera consists of 779 amino acids and has a calculated molecular mass of 88.5 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.

> 80 % as determined by SDS-PAGE

The specific activity was determined to be 35 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.