Tyr-PDGF A-Chain (194-211) peptide

Tyr-PDGF A-Chain (194-211) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

YGRPRESGKKRKRKRLKPT

H-Tyr-Gly-Arg-Pro-Arg-Glu-Ser-Gly-Lys-Lys-Arg-Lys-Arg-Lys-Arg-Leu-Lys-Pro-Thr-OH

19

95.8%

C₁₀₁H₁₈₁N₃₉O₂₅

2341.8

Synthetic

Powder

Alternative splicing of the platelet-derived growth factor A-chain RNA gives rise to two distinct A-chain forms that differ in the presence or absence of a hydrophilic carboxy-terminus by 18 amino acids (residues 194-211). H-8335, which corresponds to this region and is N-terminally tyrosinated for convenient radioiodination, was shown to bind specifically to several normal and transformed cell lines. Furthermore, this peptide and its non-tyrosinated homolog (H-2736) inhibit the stimulation of D

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• L.M.Khachigian and C.N.Chesterman, J. Biol. Chem., 267, 7478 (1992)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.