Product Name

TXNRD1 antibody

Documents

Description

TXNRD1 Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human testis tissue. Positive WB detected in Jurkat cells, A549 cells, human brain tissue, human heart tissue, human liver tissue, MCF7 cells, mouse heart tissue. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 55 kDa

Tested applications

ELISA, WB, IHC, IF, FC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

GRIM 12 antibody; GRIM12 antibody; KDRF antibody; thioredoxin reductase 1 antibody; Thioredoxin reductase TR1 antibody; TR1 antibody; TRXR1 antibody; TXNR antibody; TXNRD1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of TXNRD1 recombinant protein (Accession Number: NM_182729). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116458(TXNRD1 antibody) at dilution of 1:500

IP Result of anti-TXNRD1 (IP:Catalog No:116458, 3ug; Detection:Catalog No:116458 1:500) with Jurkat cells lysate 1500ug.

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116458(TXNRD1 antibody) at dilution of 1:100 (under 10x lens)

Immunofluorescent analysis of MCF-7 cells, using TXNRD1 antibody Catalog No:116458 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 MCF-7 cells were stained with 0.2ug TXNRD1 antibody (Catalog No:116458, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Background

TXNRD1(Thioredoxin reductase 1, cytoplasmic) is also named as GRIM12, KDRF and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.Thioredoxin reductase is a key enzyme in the regulation of the intracellular redox environment.The human TXNRD1 gene is located on chromosome 12 and encodes aprotein of 495 amino acids with a molecular mass of 54.1kDa(PMID:11737861).The protein has 6 isoforms produced by alternative splicing.

References

Miniard AC, Middleton LM, Budiman ME, Gerber CA, Driscoll DM. Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression. Nucleic acids research. 38(14):4807-20. 2010.
Zhu X, Huang C, Peng B. Overexpression of thioredoxin system proteins predicts poor prognosis in patients with squamous cell carcinoma of the tongue. Oral oncology. 47(7):609-14. 2011.
Iwasawa S, Yamano Y, Takiguchi Y, Tanzawa H, Tatsumi K, Uzawa K. Upregulation of thioredoxin reductase 1 in human oral squamous cell carcinoma. Oncology reports. 25(3):637-44. 2011.
Reinke EN, Ekoue DN, Bera S, Mahmud N, Diamond AM. Translational regulation of GPx-1 and GPx-4 by the mTOR pathway. PloS one. 9(4):e93472. 2014.
Bera S, Weinberg F, Ekoue DN. Natural allelic variations in glutathione peroxidase-1 affect its subcellular localization and function. Cancer research. 74(18):5118-26. 2014.
Brown-Borg HM, Rakoczy S, Wonderlich JA, Armstrong V, Rojanathammanee L. Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice. Longevity & healthspan. 3(1):10. 2014.

TXNRD1 antibody

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