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Product Name
TXNIP antibody
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Description
TXNIP Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, human stomach tissue, PC-12 cells. Positive IP detected in K-562 cells. Positive IHC detected in human kidney tissue, human lung tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IHC, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EST01027 antibody; HHCPA78 antibody; THIF antibody; Thioredoxin binding protein 2 antibody; TXNIP antibody; VDUP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TXNIP recombinant protein (Accession Number: NM_006472). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116454(TXNIP Antibody) at dilution of 1:600
IP Result of anti-TXNIP (IP:Catalog No:116454, 4ug; Detection:Catalog No:116454 1:500) with K-562 cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:116454(TXNIP Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:116454(TXNIP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116454(TXNIP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TXNIP represents thioredoxin interacting protein
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"