-
Product Name
TXNDC4 antibody
- Documents
-
Description
TXNDC4 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HeLa cells, HepG2 cells, MCF7 cells. Positive IP detected in K-562 cells. Positive IF detected in Hela cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 44-47 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human; other species not tested.
-
Alternative names
ER protein 44 antibody; ERP44 antibody; KIAA0573 antibody; PDIA10 antibody; TXNDC4 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TXNDC4 recombinant protein (Accession Number: NM_015051). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116451(ERP44 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116451(ERP44 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116451(ERP44 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ERP44 antibody Catalog No:116451 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-ERP44 (IP:Catalog No:116451, 4ug; Detection:Catalog No:116451 1:500) with K-562 cells lysate 4000ug.
-
Background
TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.
-
References
- Anelli T, Bergamelli L, Margittai E. Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM). Antioxidants & redox signaling. 16(10):1077-87. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"