Human TNFR1/CD120a/TNFRSF1A (His Tag) recombinant protein

Human TNFR1/CD120a/TNFRSF1A (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Tumor necrosis factor receptor superfamily member 1A

P55; TNF-R-I; MGC19588; FPF; CD120A; TNFAR; P55-R; TNF-R55; TNFR60; TBP1; TNF-R; TNFR1; TNFRSF1A; P60; TNFR55

P19438

TNFRSF1A; TNFAR; TNFR1

Human Cells

A DNA sequence encoding the human TNFRI (NP_001056.1) extracellular domain (Met 1-Thr 211) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human TNFRI consists of 201 amino acids after removal of the signal peptide and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRI is 30-35 kDa in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC₅₀ of biotinylated human TNFα is 5-12 μg/mL.
2. Measured by its ability to inhibit the TNFα mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 10-100 ng/mL in the presence of 0.25 ng/mL recombinant human TNFα.