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Product Name
Rat TNF-alpha/TNFA recombinant protein
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Description
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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Protein name
Tumor necrosis factor
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Protein short names
DADB-70P7.1; TNF; TNFSF1A; DIF; MGC151434; TNFSF2; TNFA; TNF-A; TNF-ALPHA; TNFALPHA
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Uniprot ID
P16599
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Gene Name
Tnf; Tnfa; Tnfsf2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mature form of rat TNF? (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus.
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Protein Species
Rat
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Molecular weight
The recombinant rat TNF? consisting of 157 amino acids and has a calculated molecular mass of 17.4 kDa. It migrates as an approximately 15 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 93 % as determined by SDS-PAGE
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Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 0.01-0.02 ng/mL. -
Validations
Rat TNF-alpha / TNFA Protein SDS-PAGE
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