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Product Name
Tubulin Beta 2 Monoclonal Antibody
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Description
Monoclonal antibody to Tubulin Beta 2
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Tubulin Beta 2.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50 -
Validations
Western blot - Tubulin Beta 2 Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using Tubulin Beta 2 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - Tubulin Beta 2 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using Tubulin Beta 2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Tubulin Beta 2 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using Tubulin Beta 2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Tubulin Beta 2 Monoclonal Antibody
Immunofluorescence analysis of N2A cells using Tubulin Beta 2 antibody .
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Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"