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Product Name
TUBB8 Polyclonal Antibody
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Description
Polyclonal antibody to TUBB8
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TUBB8 antibody; OOMD antibody; OOMD2 antibody; bA631M21.2 antibody; tubulin beta-8 chain antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-444 of human TUBB8 (NP_817124.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - TUBB8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TUBB8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - TUBB8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using TUBB8 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TUBB8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using TUBB8 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TUBB8 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TUBB8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). TUBB8 has a key role in meiotic spindle assembly and oocyte maturation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"