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Product Name
TTL antibody
- Documents
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Description
TTL Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, HeLa cells, human brain tissue, mouse brain tissue, rat brain tissue, Y79 cells. Observed molecular weight by Western-blot: 43-45 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
TTL antibody; Tubulin tyrosine ligase antibody; tubulin tyrosine ligase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TTL recombinant protein (Accession Number: NM_153712). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:116480(TTL antibody) at dilution of 1:1000
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Background
TTL represents tubulin tyrosine ligase
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References
- Zink S, Grosse L, Freikamp A, Bänfer S, Müksch F, Jacob R. Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells. Journal of cell science. 125(Pt 24):5998-6008. 2012.
- Song W, Cho Y, Watt D, Cavalli V. Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated α-Tubulin in Injured Axons Is Required for Retrograde Injury Signaling and Axon Regeneration. The Journal of biological chemistry. 290(23):14765-75. 2015.
- Herms A, Bosch M, Reddy BJ. AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. Nature communications. 6:7176. 2015.
- Barisic M, Silva e Sousa R, Tripathy SK. Mitosis. Microtubule detyrosination guides chromosomes during mitosis. Science (New York, N.Y.). 348(6236):799-803. 2015.
- Prota AE, Magiera MM, Kuijpers M. Structural basis of tubulin tyrosination by tubulin tyrosine ligase. The Journal of cell biology. 200(3):259-70. 2013.
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