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Product Name
TTF2 antibody
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Description
TTF2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human ovary tumor tissue. Positive WB detected in A2780 cells. Positive IP detected in A2780 cells. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HuF2 antibody; Lodestar homolog antibody; Transcription release factor 2 antibody; TTF2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TTF2 recombinant protein (Accession Number: BC030058). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:116477(TTF2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116477(TTF2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TTF2 antibody Catalog No:116477 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-TTF2 (IP:Catalog No:116477, 4ug; Detection:Catalog No:116477 1:500) with A2780 cells lysate 840ug.
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Background
Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].
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References
- Brannan K, Kim H, Erickson B. mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription. Molecular cell. 46(3):311-24. 2012.
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