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Product Name
TTBK2 Polyclonal Antibody
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Description
Polyclonal antibody to TTBK2
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Tested applications
WB, IHC
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Species reactivity
Human, Rat
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Alternative names
TTBK2 antibody; SCA11 antibody; TTBK antibody; tau-tubulin kinase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1185-1244 of human TTBK2 (NP_775771.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - TTBK2 Polyclonal Antibody
Western blot analysis of extracts of rat brain, using TTBK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - TTBK2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using TTBK2 antibody at dilution of 1:200 (40x lens).
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Background
Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"