-
Product Name
TRPV5 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TRPV5
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
TRPV5 antibody; CAT2 antibody; ECAC1 antibody; OTRPC3 antibody; transient receptor potential cation channel subfamily V member 5 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human TRPV5 (NP_062815.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - TRPV5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRPV5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - TRPV5 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TRPV5 antibody .
-
Background
Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. Required for normal Ca(2+) reabsorption in the kidney distal convoluted tubules (By similarity). The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay (By similarity). Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"