-
Product Name
TRPV2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TRPV2
-
Tested applications
WB
-
Species reactivity
Human
-
Alternative names
TRPV2 antibody; VRL antibody; VRL-1 antibody; VRL1 antibody; transient receptor potential cation channel subfamily V member 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 655-764 of human TRPV2 (NP_057197.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - TRPV2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRPV2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
-
Background
Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"