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Product Name
TrpRS antibody
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Description
TrpRS Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells. Positive FC detected in HepG2 cells. Positive IHC detected in human ovary tumor tissue, human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 53kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GAMMA 2 antibody; hWRS antibody; IFI53 antibody; IFP53 antibody; Interferon induced protein 53 antibody; TrpRS antibody; Tryptophan tRNA ligase antibody; Tryptophanyl-tRNA synthetase antibody; WARS antibody; WRS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TrpRS recombinant protein (Accession Number: XM_017021627). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116413(WARS antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116413(WARS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116413(WARS antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using WARS antibody Catalog No:116413 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug WARS antibody (Catalog No:116413, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing.
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