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Product Name
TRPC4AP antibody
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Description
TRPC4AP Rabbit Polyclonal antibody. Positive WB detected in human heart tissue. Positive IP detected in mouse heart tissue. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 91 kDa
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Tested applications
ELISA, IP, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C20orf188 antibody; Protein TAP1 antibody; Protein TRUSS antibody; Trp4 assOCiated protein antibody; Trpc4 assOCiated protein antibody; TRPC4AP antibody; TRRP4AP antibody; TRUSS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRPC4AP recombinant protein (Accession Number: XM_047440095). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
IP Result of anti-TRPC4AP (IP:Catalog No:116407, 5ug; Detection:Catalog No:116407 1:200) with mouse heart tissue lysate 6000ug.
Immunohistochemistry of paraffin-embedded human prostate cancer slide using Catalog No:116407(TRPC4AP Antibody) at dilution of 1:50
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:116407(TRPC4AP Antibody) at dilution of 1:300
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Background
TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.
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References
- Mannava S, Omilian AR, Wawrzyniak JA. PP2A-B56α controls oncogene-induced senescence in normal and tumor human melanocytic cells. Oncogene. 31(12):1484-92. 2012.
- Jamal A, Swarnalatha M, Sultana S, Joshi P, Panda SK, Kumar V. The G1 phase E3 ubiquitin ligase TRUSS that gets deregulated in human cancers is a novel substrate of the S-phase E3 ubiquitin ligase Skp2. Cell cycle (Georgetown, Tex.). 14(16):2688-700. 2015.
- Choi SH, Wright JB, Gerber SA, Cole MD. Myc protein is stabilized by suppression of a novel E3 ligase complex in cancer cells. Genes & development. 24(12):1236-41. 2010.
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