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Product Name
TRMT6 antibody
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Description
TRMT6 Rabbit Polyclonal antibody. Positive IHC detected in human oesophagus tissue, human testis tissue. Positive WB detected in human brain tissue, mouse ovary tissue. Observed molecular weight by Western-blot: 56 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 09 antibody; GCD10 antibody; Gcd10p antibody; KIAA1153 antibody; TRM6 antibody; TRMT6 antibody; tRNA(m1A58)MTase subunit TRM6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRMT6 recombinant protein (Accession Number: NM_015939). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116399(TRMT6 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human oesophagus tissue slide using Catalog No:116399(TRMT6 Antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116399(TRMT6 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein.
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