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Product Name
TRMT1 antibody
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Description
TRMT1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 72 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20244 antibody; TRM1 antibody; TRMT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRMT1 recombinant protein (Accession Number: XM_017026944). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116395(TRMT1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116395(TRMT1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116395(TRMT1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116395(TRMT1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TRMT1 represents TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"