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Product Name
TRIT1 Polyclonal Antibody
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Description
Polyclonal antibody to TRIT1
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Tested applications
WB
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Species reactivity
Human
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Alternative names
TRIT1 antibody; GRO1 antibody; IPPT antibody; IPT antibody; IPTase antibody; MOD5 antibody; hGRO1 antibody; tRNA isopentenyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 48-300 of human TRIT1 (NP_060116.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - TRIT1 Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using TRIT1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
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Background
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"