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Product Name
TRIM69 Polyclonal Antibody
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Description
Polyclonal antibody to TRIM69
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TRIM69 antibody; HSD-34 antibody; HSD34 antibody; RNF36 antibody; Trif antibody; tripartite motif containing 69 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TRIM69 Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using TRIM69 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - TRIM69 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using TRIM69 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TRIM69 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using TRIM69 antibody . Blue: DAPI for nuclear staining.
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Background
May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"