-
Product Name
TRIM45 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TRIM45
-
Tested applications
WB
-
Species reactivity
Human, Mouse
-
Alternative names
TRIM45 antibody; RNF99 antibody; tripartite motif containing 45 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM45 (NP_001139107.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - TRIM45 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRIM45 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"