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Product Name
TRIM31 antibody
- Documents
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Description
TRIM31 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IP detected in COLO 320 cells. Positive WB detected in COLO 320 cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
C6orf13 antibody; HCG1 antibody; HCGI antibody; RNF antibody; TRIM31 antibody; tripartite motif containing 31 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM31 recombinant protein (Accession Number: NM_007028). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:116310(TRIM31 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116310(TRIM31 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-TRIM31 (IP:Catalog No:116310, 4ug; Detection:Catalog No:116310 1:400) with COLO 320 cells lysate 3200ug.
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Background
TRIM31(Tripartite motif-containing protein 31) is also named as C6orf13, RNF and belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING domain, a B-box-2 domain, and a coiled-coil region. TRIM31 forms homodimers(PMID:11331580). This protein has 2 isoforms produced by alternative splicing.
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References
- Uchil PD, Hinz A, Siegel S. TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity. Journal of virology. 87(1):257-72. 2013.
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