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Product Name
TRIM27 Polyclonal Antibody
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Description
Polyclonal antibody to TRIM27
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
TRIM27 antibody; RFP antibody; RNF76 antibody; zinc finger protein RFP antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 214-513 of human TRIM27 (NP_006501.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - TRIM27 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRIM27 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - TRIM27 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRIM27 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - TRIM27 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using TRIM27 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TRIM27 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using TRIM27 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TRIM27 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TRIM27 antibody . Blue: DAPI for nuclear staining.
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Background
E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"