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Product Name
TRIM27 antibody
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Description
TRIM27 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in HeLa cells. Positive WB detected in Jurkat cells, human testis tissue, mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Ret finger protein antibody; RFP antibody; RING finger protein 76 antibody; RNF76 antibody; TRIM27 antibody; tripartite motif containing 27 antibody; Zinc finger protein RFP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM27 recombinant protein (Accession Number: NM_006510). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116308(TRIM27 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116308(TRIM27 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-TRIM27 (IP:Catalog No:116308, 4ug; Detection:Catalog No:116308 1:1000) with mouse testis tissue lysate 4000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:116308(TRIM27 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TRIM27 represents tripartite motif-containing 27
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References
- Ma Y, Wei Z, Bast RC. Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo. Laboratory investigation; a journal of technical methods and pathology. 96(1):37-48. 2016.
- Nie D, Zhang D, Dai J. Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. Biology of reproduction. 94(2):31. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"