-
Product Name
TRIM25 antibody
- Documents
-
Description
TRIM25 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in MCF7 cells, Jurkat cells, rat spleen tissue. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 71 kDa
-
Tested applications
ELISA, WB, IF, IP
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
EFP antibody; RING finger protein 147 antibody; RNF147 antibody; TRIM25 antibody; tripartite motif containing 25 antibody; Z147 antibody; Zinc finger protein 147 antibody; ZNF147 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TRIM25 recombinant protein (Accession Number: NM_005082). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
-
Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116307(TRIM25 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using TRIM25 antibody Catalog No:116307 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-TRIM25 (IP:Catalog No:116307, 4ug; Detection:Catalog No:116307 1:600) with K-562 cells lysate 3200ug.
-
Background
TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790).
-
References
- Wang P, Arjona A, Zhang Y. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nature immunology. 11(10):912-9. 2010.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"