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Product Name
TRIM24 antibody
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Description
TRIM24 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in HeLa cells, BGC-823 cells, human heart tissue, L02 cells, MCF7 cells, mouse skeletal muscle tissue. Positive IF detected in HeLa cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 116 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hTIF1 antibody; PTC6 antibody; RING finger protein 82 antibody; RNF82 antibody; TF1A antibody; TIF1 antibody; TIF1 alpha antibody; TIF1A antibody; TIF1ALPHA antibody; TRIM24 antibody; tripartite motif containing 24 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM24 recombinant protein (Accession Number: NM_015905). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116306(TRIM24 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:116306(TRIM24 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:116306(TRIM24 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-TRIM24 (IP:Catalog No:116306, 4ug; Detection:Catalog No:116306 1:800) with MCF-7 cells lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:116306(TRIM24 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.
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References
- Li H, Sun L, Tang Z. Overexpression of TRIM24 correlates with tumor progression in non-small cell lung cancer. PloS one. 7(5):e37657. 2012.
- Cui Z, Cao W, Li J, Song X, Mao L, Chen W. TRIM24 overexpression is common in locally advanced head and neck squamous cell carcinoma and correlates with aggressive malignant phenotypes. PloS one. 8(5):e63887. 2013.
- Pathiraja TN, Thakkar KN, Jiang S. TRIM24 links glucose metabolism with transformation of human mammary epithelial cells. Oncogene. 34(22):2836-45. 2015.
- Miao ZF, Wang ZN, Zhao TT. TRIM24 is upregulated in human gastric cancer and promotes gastric cancer cell growth and chemoresistance. Virchows Archiv : an international journal of pathology. 466(5):525-32. 2015.
- Xue D, Zhang X, Zhang X. Clinical significance and biological roles of TRIM24 in human bladder carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(9):6849-55. 2015.
- Zhan Y, Kost-Alimova M, Shi X. Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery. Epigenetics & chromatin. 8:37. 2015.
- Tsai WW, Wang Z, Yiu TT. TRIM24 links a non-canonical histone signature to breast cancer. Nature. 468(7326):927-32. 2010.
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