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Product Name
TRIM22 Polyclonal Antibody
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Description
Polyclonal antibody to TRIM22
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
TRIM22 antibody; GPSTAF50 antibody; RNF94 antibody; STAF50 antibody; tripartite motif containing 22 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-411 of human TRIM22 (NP_006065.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:50 - 1:100 -
Validations
Western blot - TRIM22 Polyclonal Antibody
Western blot analysis of extracts of A-549 cells, using TRIM22 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - TRIM22 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TRIM22 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Interferon-induced antiviral protein involved in cell innate immunity. The antiviral activity could in part be mediated by TRIM22-dependent ubiquitination of viral proteins. Plays a role in restricting the replication of HIV-1, encephalomyocarditis virus (EMCV) and hepatitis B virus (HBV). Acts as a transcriptional repressor of HBV core promoter. May have E3 ubiquitin-protein ligase activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"