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Product Name
TRIM21 antibody
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Description
TRIM21 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, HeLa cells, HL-60 cells, HT-1080 cells, mouse pancreas tissue. Positive IP detected in A549 cells. Positive IHC detected in human lung cancer tissue. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
52 kDa Ro protein antibody; RING finger protein 81 antibody; RNF81 antibody; Ro(SS A) antibody; RO52 antibody; SS A antibody; SSA antibody; SSA1 antibody; TRIM21 antibody; tripartite motif containing 21 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM21 recombinant protein (Accession Number: NM_003141). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:116303(TRIM21 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116303(TRIM21 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-TRIM21 (IP:Catalog No:116303, 4ug; Detection:Catalog No:116303 1:500) with A549 cells lysate 920ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using Catalog No:116303(TRIM21 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules.
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References
- Shibata N, Ohoka N, Sugaki Y. Degradation of Stop Codon Read-through Mutant Proteins via the Ubiquitin-Proteasome System Causes Hereditary Disorders. The Journal of biological chemistry. 290(47):28428-37. 2015.
- Fan W, Zhang D, Qian P. Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism. Antiviral research. 127:32-40. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"