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Product Name
TRIM2 antibody
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Description
TRIM2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human kidney tissue. Positive WB detected in mouse small intestine tissue, mouse brain tissue. Observed molecular weight by Western-blot: 70-82kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0517 antibody; RING finger protein 86 antibody; RNF86 antibody; TRIM2 antibody; tripartite motif containing 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRIM2 recombinant protein (Accession Number: XM_017007950). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:116302(TRIM2 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:116302(TRIM2 Antibody) at dilution of 1:50(under 40x lens)
Immunofluorescent analysis of Hela cells, using TRIM2 antibody Catalog No:116302 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.
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