TRHR Polyclonal Antibody

Cat.#: 162523

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Product Information

  • Product Name
    TRHR Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to TRHR
  • Tested applications
    WB
  • Species reactivity
    Human
  • Alternative names
    TRHR antibody; TRH-R antibody; thyrotropin-releasing hormone receptor antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-398 of human TRHR (NP_003292.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - TRHR Polyclonal Antibody

    Western blot - TRHR Polyclonal Antibody

    Western blot analysis of extracts of U-87MG cells, using TRHR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.

  • Background
    Receptor for thyrotropin-releasing hormone. This receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"