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Product Name
TREM1 antibody
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Description
TREM1 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, A549 cells, HepG2 cells, human liver tissue, human spleen tissue, mouse lung tissue. Positive IF detected in HepG2 cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IF, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CD354 antibody; TREM 1 antibody; TREM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TREM1 recombinant protein (Accession Number: NM_018643). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116356(TREM1 antibody) at dilution of 1:400
Immunofluorescent analysis of HepG2 cells, using TREM1 antibody Catalog No:116356 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HeLa cells were stained with 0.2ug TREM1 antibody (Catalog No:116356, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Triggering receptor expressed on myeloid cells 1 (TREM1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"