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Product Name
Trefoil factor 1 antibody
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Description
Trefoil factor 1 Rabbit Polyclonal antibody. Positive IHC detected in human stomach cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in human stomach tissue, human kidney tissue. Observed molecular weight by Western-blot: 9 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
D21S21 antibody; HP1.A antibody; HPS2 antibody; pNR 2 antibody; Polypeptide P1.A antibody; Protein pS2 antibody; pS2 antibody; TFF1 antibody; trefoil factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Trefoil factor 1 recombinant protein (Accession Number: NM_003225). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:116354(TFF1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:116354(TFF1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:116354(TFF1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116354(TFF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TFF1 (formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.
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References
- Zhao S, Ma Y, Huang X. Trefoil factor 1 elevates the malignant phenotype of mucinous ovarian cancer cell through Wnt/β-catenin signaling. International journal of clinical and experimental pathology. 8(9):10412-9. 2015.
- Kim HK, Kim J, Korolevich S. Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection. BMC medical genomics. 4:48. 2011.
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