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Product Name
TRBP antibody
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Description
TRBP Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in HepG2 cells, mouse liver tissue, mouse spleen tissue. Positive IF detected in Hela cells. Positive IHC detected in human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 43-46 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
LOQS antibody; TAR RNA binding protein 2 antibody; TARBP2 antibody; TRBP antibody; TRBP1 antibody; TRBP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRBP recombinant protein (Accession Number: NM_134323). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116352(TRBP antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:116352(TRBP antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TARBP2 antibody Catalog No:116352 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TRBP (IP:Catalog No:116352, 4ug; Detection:Catalog No:116352 1:1500) with mouse liver tissue lysate 6400ug.
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Background
TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs.
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References
- Chen C, Zhu C, Huang J. SUMOylation of TARBP2 regulates miRNA/siRNA efficiency. Nature communications. 6:8899. 2015.
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