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Product Name
TRAP1 Polyclonal Antibody
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Description
Polyclonal antibody to TRAP1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TRAP1 antibody; HSP 75 antibody; HSP75 antibody; HSP90L antibody; TRAP-1 antibody; TNF receptor associated protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human TRAP1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TRAP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - TRAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - TRAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TRAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TRAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TRAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TRAP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using TRAP1 Antibody at dilution of 1:100 (40x lens).
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Background
Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"