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Product Name
Transferrin receptor/CD71 antibody
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Description
Transferrin receptor/CD71 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human lymphoma tissue. Positive IP detected in HeLa cells. Positive WB detected in MCF7 cells, HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CD71 antibody; p90 antibody; sTfR antibody; TFR antibody; TFR1 antibody; TFRC antibody; Transferrin receptor antibody; Transferrin receptor protein 1 antibody; TRFR antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Transferrin receptor/CD71 recombinant protein (Accession Number: NM_001128148). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116331(CD71 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116331(CD71 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116331(CD71 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CD71 (IP:Catalog No:116331, 4ug; Detection:Catalog No:116331 1:500) with HeLa cells lysate 2400ug.
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Background
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
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References
- Lacruz RS, Brookes SJ, Wen X. Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 28(3):672-87. 2013.
- Liang B, Peng P, Chen S. Characterization and proteomic analysis of ovarian cancer-derived exosomes. Journal of proteomics. 80:171-82. 2013.
- Ariza J, Steward C, Rueckert F. Dysregulated iron metabolism in the choroid plexus in fragile X-associated tremor/ataxia syndrome. Brain research. 1598:88-96. 2015.
- Giorgi G, D'Anna MC, Roque ME. Iron homeostasis and its disruption in mouse lung in iron deficiency and overload. Experimental physiology. 100(10):1199-216. 2015.
- Wang X, Zhu N, Li W, Zhu F, Wang Y, Yuan Y. Mono-ubiquitylated ORF45 Mediates Association of KSHV Particles with Internal Lipid Rafts for Viral Assembly and Egress. PLoS pathogens. 11(12):e1005332. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"