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Product Name
TPX2 Polyclonal Antibody
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Description
Polyclonal antibody to TPX2
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
TPX2 antibody; C20orf1 antibody; C20orf2 antibody; DIL-2 antibody; DIL2 antibody; FLS353 antibody; GD:C20orf1 antibody; HCA519 antibody; HCTP4 antibody; REPP86 antibody; p100 antibody; targeting protein for Xklp2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human TPX2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - TPX2 Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using TPX2 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"