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Product Name
TPX2 antibody
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Description
TPX2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, K-562 cells. Positive IP detected in HeLa cells. Positive IHC detected in human prostate cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C20orf1 antibody; C20orf2 antibody; DIL 2 antibody; DIL2 antibody; FLS353 antibody; GD:C20orf1 antibody; HCA519 antibody; HCTP4 antibody; p100 antibody; Protein fls353 antibody; REPP86 antibody; Targeting protein for Xklp2 antibody; TPX2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TPX2 recombinant protein (Accession Number: NM_012112). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116223(TPX2 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using TPX2 antibody Catalog No:116223 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TPX2 (IP:Catalog No:116223, 4ug; Detection:Catalog No:116223 1:800) with HeLa cells lysate 880ug.
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:116223(TPX2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:116223(TPX2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
TPX2 represents TPX2, microtubule-associated, homolog (Xenopus laevis)
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References
- Hu MW, Wang ZB, Teng Y. Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility. Journal of cell science. 128(20):3769-80. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"