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Product Name
TPSAB1 Polyclonal Antibody
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Description
Polyclonal antibody to TPSAB1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
TPSAB1 antibody; TPS1 antibody; TPS2 antibody; TPSB1 antibody; tryptase alpha/beta-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-210 of human TPSAB1 (NP_003285.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:20 - 1:50 -
Validations
Western blot - TPSAB1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TPSAB1 antibody at 1:200 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - TPSAB1 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using TPSAB1 antibody . Blue: DAPI for nuclear staining.
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Background
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"