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TPPP3 antibody

Cat.#: 116214

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Special Price 239.0 USD

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Product Information

  • Product Name
    TPPP3 antibody
  • Documents
  • Description
    TPPP3 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, HeLa cells. Observed molecular weight by Western-blot: 19-20 kDa
  • Tested applications
    ELISA, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    TPPP3 antibody; TPPP/p20 antibody; CGI 38 antibody; p25gamma antibody; p20 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TPPP3 recombinant protein (Accession Number: NM_016140). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

  • Validations

    WB result of anti-TPPP3 (Catalog No:116214, 1:500) with HEK-293 cells (siRNA transfected with) by Dr. Yupeng Chen. (Negative control or TPPP3 specific siRNAs were transfected into HEK-293 cells with the final concentration of 20 nM. Forty eight hours after transfection, cells were lysed with RIPA buffer and subjected to SDS PAGE)

    WB result of anti-TPPP3 (Catalog No:116214, 1:500) with HEK-293 cells (siRNA transfected with) by Dr. Yupeng Chen. (Negative control or TPPP3 specific siRNAs were transfected into HEK-293 cells with the final concentration of 20 nM. Forty eight hours after transfection, cells were lysed with RIPA buffer and subjected to SDS PAGE)

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116214(TPPP3 antibody) at dilution of 1:100

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116214(TPPP3 antibody) at dilution of 1:100

  • Background
    TPPP3 (tubulin polymerization promoting protein 3), also named as TPPP/p20, is a new member of the TPPP family which binds tubulin and induces tubulin polymerization and microtubule bundling.TPPP3 has been shown to play an important role in cell proliferation and may be implicated in tumorigenesis and metastasis (19633818). TPPP3 was also identified as specific marker for the connective tissues that envelope tendons due to its distinct expression in the developing tendon sheath (19235716). This antibody detects the 19-20 kDa TPPP3 in HeLa and PC-3 cells. The non-specific bands between 40 and 60 kDa were also occasionally observed.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"