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Product Name
TPMT antibody
- Documents
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Description
TPMT Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in HEK-293 cells. Positive WB detected in human kidney tissue, HeLa cells, mouse kidney tissue. Observed molecular weight by Western-blot: 28-35kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Thiopurine methyltransferase antibody; thiopurine S methyltransferase antibody; TPMT antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TPMT recombinant protein (Accession Number: NM_001346817). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116208(TPMT antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116208(TPMT antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:116208(TPMT Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. This antibody specially recognises the 34 kDa protein.
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