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Product Name
TPM2 antibody
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Description
TPM2 Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue. Positive IP detected in mouse skeletal muscle tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human colon tissue, human heart tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 29-33 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMCD1 antibody; Beta tropomyosin antibody; DA1 antibody; DA2B antibody; TMSB antibody; TPM2 antibody; Tropomyosin 2 antibody; tropomyosin 2 (beta) antibody; Tropomyosin beta chain antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TPM2 recombinant protein (Accession Number: NM_213674). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:116267(TPM2 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116267(TPM2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116267(TPM2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TPM2 antibody Catalog No:116267 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-TPM2 (IP:Catalog No:116267, 4ug; Detection:Catalog No:116267 1:500) with mouse skeletal muscle tissue lysate 3600ug.
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Background
TPM2, also named as TMSB, belongs to the tropomyosin family. It binds to actin filaments in muscle and non-muscle cells. TPM2 plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. In non-piamuscle cells, TPM2 is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4) and distal arthrogryposis type 1 (DA1).
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