-
Product Name
TPD52L2 antibody
- Documents
-
Description
TPD52L2 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue, Jurkat cells, K-562 cells, MCF7 cells, mouse brain tissue, rat brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human testis tissue. Observed molecular weight by Western-blot: 25-30kd
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
D54 antibody; DKFZp686A1765 antibody; hD54 antibody; TPD52L2 antibody; TPD54 antibody; tumor protein D52 like 2 antibody; Tumor protein D54 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TPD52L2 recombinant protein (Accession Number: NM_003288). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116262(TPD52L2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116262(TPD52L2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TPD52L2 antibody Catalog No:116262 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TPD52L2 (IP:Catalog No:116262, 3ug; Detection:Catalog No:116262 1:800) with HEK-293 cells lysate 2400ug.
-
Background
Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"