-
Product Name
TOPBP1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TOPBP1
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
TOPBP1 antibody; TOP2BP1 antibody; DNA topoisomerase 2-binding protein 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOPBP1 (NP_008958.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - TOPBP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TOPBP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - TOPBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using TOPBP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TOPBP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using TOPBP1 antibody at dilution of 1:100 (40x lens).
-
Background
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"